Revised ISO 11133 and ISO 16649 Food Microbiology Standards - How This Affects Your Lab.


Format: External webinar | Document type: Webinar


This webinar explains the amendments of the ISO 11133:2014 and the revised ISO 16649 part 1:2018 for enumeration of Escherichia coli in the food chain. 
ISO 11133:2014 is a mandatory standard for all ISO 17025-accredited labs for microbiological analysis of food, feed and water. It describes the preparation, production and performance testing of culture media from this whole area. Recently published amendment 1:2018 contains corrections and additional explanations whilst future amendment 2 will describe the performance testing of confirmation media and reagents. 
ISO 16649-1, which specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli, has also been revised. The method uses a colony-count technique after resuscitation using membranes and incubation at 44 °C on TBX agar. 
There are three horizontal methods (ISO 16649-1, ISO 16649-2 and ISO 16649-3) for the enumeration of β-glucuronidase-positive Escherichia coli, all parts are for general application. Parts 1 and 3 include a resuscitation step and should be used in preference for foodstuffs likely to contain sub-lethally injured cells. 
This webinar explains the latest changes in ISO 11133 and ISO 16649 and will give an outlook on the future of both standards.

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Barbara Gerten Barbara Gerten Senior Scientist Traditional Microbiology
Merck KGaA, Darmstadt, Germany

Dr. Andreas Bubert Andreas Bubert Senior Product Manager Culture Media Food & Beverage
Merck KGaA, Darmstadt, Germany

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