Researchers will use the Kapa Library Preparation Kit to construct DNA fragment libraries for next-generation sequencing on Illumina platforms to unlock the genomes of Salmonella, Listeria, and E. coli.
The KAPA kit employs formulated and enzymes combined with a protocol to achieve diverse libraries and improved sequence quality.
The US Food and Drug Administration (FDA), University of California, Davis, Agilent Technologies and the Centers for Disease Control and Prevention (CDC) are involved in the project.
The five-year effort will create the largest public database of 100,000 foodborne pathogen genomes to speed identification of bacteria responsible for outbreaks and reduce public health response time from weeks to days using next-generation sequencing platforms.
Bart Weimer, project director and Professor of population health & reproduction, school of veterinary medicine at UC Davis, said: “Microbes by nature can be difficult to sequence as they often contain a high percentage of AT- and GC-rich genomic content.
“Uniform sequencing coverage is crucial to building a high quality genomic database, and it is important that we use reagents that consistently produce low-bias, high-yield libraries that enable us to maximize the genomic information we obtain from each sample.”